On 2nd May the Commission released the much-awaited Guidance on how to test for nanomaterials following the new recommendation of a definition (2022/C 229/01) adopted by the European Commission in 2022. The guidance builds from the two JRC Science for Policy Reports EUR 29647 and EUR 29942 and provides an overview of key terms and concepts, a decision tree to identify nanomaterials and an Annex with documentary standards relevant for a harmonised and coherent regulatory implementation of the definition.